Ligands Identified by Physical Screening

Porphobilinogen synthase (Tgon007037) Ligands

The intensity of a fluorescent dye in the presence of protein is plotted as a function of temperature. As the dye binds to unfolded protein, its fluorescence increases. The melting temperature of the protein is defined as the temperature at which the fluorescent intensity is increasing most rapidly. A small molecule that stabilizes the protein will increase the melting temperature of the protein. In the following charts, intensity is normalized to the maximum intensity.

Compound 4197