MSGPP | 3-D Structures

Plasmodium falciparum
	Pfal008434AAA Plasmodium falciparum Ribose 5-phosphate isomerase [ PDB-ID 2F8M ]
	Pfal000304AAA Plasmodium falciparum Orotidine-5'-monophosphate decarboxylase [ PDB-ID 2F84 ]
	Pfal007254AAA Plasmodium falciparum Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme. This target has been an exploratory target for drug design by the MMV (Medicines for Malaria Venture: http://www.mmv.org). Shown in CPK format are 4 copies of the NAD cofactor found in the 2.25 Angstrom homotetrameric crystal structure. [ PDB-ID 2B4R ]
	Pfal009167AAA Plasmodium falciparum D-Ribulose 5-Phosphate 3-Epimerase Enzyme from the oxidative pentose phosphate pathway (critical for the generation of NADPH) [ PDB-ID 1TQX ]
	Pfal008421AAA Plasmodium falciparum Homolog of Uridine Phosphorylase/Purine Nucleoside Phosphorylase [ PDB-ID 1SQ6 ]
	Pfal009132AAA Plasmodium falciparum NAD-dependent Glycerol-3-Phosphate Dehydrogenase [ PDB-ID 1YJ8 ]
	Pfal006645AAA Plasmodium falciparum Nucleoside diphosphate kinase B [ PDB-ID 1XIQ ]
	Pfal004546AAA Plasmodium falciparum 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) [ PDB-ID 1Y13 ]
	Pfal005984AAA Plasmodium falciparum Phosphoglycerate mutase Metabolic enzyme; potential antimalarial drug target [ PDB-ID 1XQ9 ] [ PDB-ID 3KKK ]
Plasmodium vivax
	Pviv003883AAE Plasmodium vivax 6-phosphogluconolactonase [ PDB-ID 3E15 ]
	Pviv004546AAA Plasmodium vivax 6-pyruvoyl tetrahydrobiopterin synthase (PTPS) [ PDB-ID 2A0S ]
	Pviv005676AAA Plasmodium vivax Adenosine deaminase [ PDB-ID 2PGF ] [ PDB-ID 2PGR ] [ PDB-ID 2QVN ]
	Pviv008894AAA Plasmodium vivax Guanylate Kinase [ PDB-ID 2QOR ]
	Pviv009166AAA Plasmodium vivax Phosphatidylethanolamine binding protein (RKIP homologue) [ PDB-ID 2GZQ ]
Plasmodium knowlesi
	Pkno008421AAA Plasmodium knowlesi Purine nucleoside phosphorylase A homolog of Pfal008421AAA. [ PDB-ID 2B94 ]
	Pkno009013AAC Plasmodium knowlesi Nucleosome assembly protein 1 [ PDB-ID 3HFD ]
Leishmania major


	Lmaj002314AAA Leishmania major Glycogen synthase kinase [ PDB-ID 3E3P ]
	Lmaj007771AAA Leishmania major Cyclophilin [ PDB-ID 2HQJ ]
	Lmaj002537BAD Leishmania major Protein tyrosine phosphatase
	Lmaj004542AAA Leishmania major Mevalonate kinase
	Lmaj005534AAA Leishmania major Eukaryotic D-Amino Acid tRNA Deacylase [ PDB-ID 1TC5 ]
	Lmaj004091AAA Leishmania major SAM dependent Methyltransferase (similar to ubiquinone MT) [ PDB-ID 1XTP ]
	Lmaj006238AAA Leishmania major Eukaryotic initiation factor 2B [ PDB-ID 2A0U ]
	Lmaj006828AAA Leishmania major Identified by structural homology to be oxygen dependent coproporphyrinogen oxidase (CPO) an essential enzyme in the heme biosynthetic pathway [ PDB-IDs 1VJU, 2QT8, 3DWR, 3DWS ] See also: L. donovani CPO [ 3EJO ], L. naiffi CPO [ 3E8J ]
	Lmaj008024AAA Leishmania major Homolog of low-speceficity Threonine Aldolase [ PDB-ID 1SVV ]
Leishmania donovani
	Ldon002030AAA Leishmania donovani Dihydroorotate dehydrogenase [ PDB-ID 3C61 ]
Leishmania mexicana
	Lmex003024AAA Leishmania mexicana Eukaryotic initiation factor 5A [ PDB-ID 1XTD ]
Leishmania guyanensis
	Lguy003394AAA Leishmania guyanensis 6-phosphogluconolactonase [ PDB-ID 3CSS ]
Leishmania braziliensis
	Lbra003394AAA Leishmania braziliensis 6-phosphogluconolactonase [ PDB-ID 3CH7 ]
	Lbra003024AAA Leishmania braziliensis Eukaryotic initiation factor 5A [ PDB-ID 1X6O ]
Leishmania naiffi
	Lnai002302AAC Leishmania naiffi Uracil DNA glycosylase [ PDB-ID 3CXM ]
Trypanosoma brucei
	Tbru001569AAB Trypanosoma brucei Tryptophanyl-tRNA synthetase [ PDB-ID 3I05 ]
	Tbru016489AAB Trypanosoma brucei Histidinyl-tRNA synthetase Dimer [ PDB-ID 3HRI ]
	Tbru022584AAA Trypanosoma brucei Seryl-tRNA synthetase Seryladenylate found in active site after soaking with serine and ATP.
	Tbru017883AAA Trypanosoma brucei Uridine phosphorylase [ PDB-ID 3BJE ]
	Tbru019101AAA Trypanosoma brucei Riboflavin kinase [ PDB-ID 3BNW ]
	Tbru020260AAA Trypanosoma brucei Unknown function [ PDB-ID 2Q0X ]
	Tbru015978AAA Trypanosoma brucei Dihydroorotate dehydrogenase [ PDB-ID 2B4G ]
	Tbru015777AAA Trypanosoma brucei Nucleoside 2-deoxyribosyltransferase [ PDB-ID 2A0K ] See also: [ 2F2T, 2F62, 2F64, 2F67, ]
Trypanosoma cruzi
	Tcru008378AAB Trypanosoma cruzi Histidinyl-tRNA synthase Dimer with histidinyl-adenylate monophosphate (HAM) bound [ PDB-ID 3HRJ ] [ PDB-ID 3HRK ]
	Tcru019078AAA Trypanosoma cruzi Spermidine synthase [ PDB-ID 3BWB ] [ PDB-ID 3BWC ]
	Tcru010945AAA Trypanosoma cruzi Arginase superfamily protein [ PDB-ID 2A0M ]
	Tcru013382AAA Trypanosoma cruzi Cyclophilin (Cyclosporin-A binding protein) Unliganded [ PDB-ID 1XO7 ]
	Tcru0013382AAA Trypanosoma cruzi Cyclophilin (Cyclosporin-A binding protein) Potential drug target PDB 1XO7 Cyclophilin complexed with known anti-Leishmanial agent Cyclosporin [ PDB-ID 1XQ7 ]
Cryptosporidum parvum
	Cpar071490AAB Cryptosporidum parvum Tryptophanyl-tRNA synthetase Dimer [ PDB-ID 3HV0 ]
Entamoeba histolytica
	Ehis058157AAB Entamoeba histolytica Tryptophanyl-tRNA synthetase Three dimers in the asymmetric unit [ PDB-ID 3hzr ]
	Ehis262059AAA Entamoeba histolytica Aspartyl-tRNA synthetase Dimers [ PDB-ID 3I7F ]
Giardia lamblia
	Gint003032AAA Giardia lamblia Tryptophanyl-tRNA synthetase Tetramer [ PDB-ID 3FOC ]
	Gint015983AAC Giardia lamblia Prolyl-tRNA synthetase Prolyladenylate and inorganic phosphate were found in the active site after cocrystallization with L-proline and ATP. [ PDB-ID 3IAL ]
Toxoplasma gondii
	Tgon065570BAB Toxoplasma gondii Calcium-dependent Protein Kinase 1 [ PDB-ID 3I79 ] [ PDB-ID 3I7B ] [ PDB-ID 3I7C ]
	Tgon077550AAB Toxoplasma gondii Cathepsin L Cysteine Proteinase Cathepsin protein L in complex with its propeptide. Protease is shown as blue ribbon; catalytic triad residues as magenta sticks; five disulfide bonds as yellow sticks; propeptide as green ribbon. The propeptide was cleaved from the protease prior to crystallization with no further purification, and remained bound. [ PDB-ID 3F75 ]

SGPP Structures not continued as targets in MSGPP

	Pfal007201AAA Plasmodium falciparum Thioredoxin [ PDB-ID 1SYR ]
	Pfal004331AAA Plasmodium falciparum hypothetical protein, conserved [ PDB-ID 1ZSO ]
	Pviv002324AAA Plasmodium vivax HAD / COF-like hydrolase Homolog to proteins belonging to the Haloacid Dehydrogenase superfamily, subfamily IIB [ PDB-ID 2B30 ]
	Pber005319AAA Plasmodium berghei Superoxide dismutase [ PDB-ID 2A03 ]
	Lmaj005461AAB Leishmania major Ubiquitin conjugating enzyme E2 [ PDB-ID 1YF9 ]
	Lmaj01134AAC Leishmania major This N-terminally truncated protein is a Leishmania major (Friedlin) homolog of the "programmed cell death 6" protein [ PDB-ID 1Y1X ]
	Lmaj001686AAA Leishmania major Putative mitochondrial associated ribonuclease (90% sequence identity to Leishmania tarentolae MAR1) high structural homology to N-carbamoylsarcosine amidohydrolase (15% sequence identity) including active site Cys [ PDB-ID 1XN4 ]
	Lmaj006238AAA Leishmania major Eukaryotic initiation factor 2B [ PDB-ID 2A0U ]
	Lmaj006873AAA Leishmania major Hypothetical protein, conserved [ PDB-ID 2B4W ]
	Lmaj011689AAA Leishmania major Homologue to human p32 protein The resolution is 2.3A [ PDB-ID 1YQF ]
	Lmaj002144AAA Leishmania major Putative calpain-like cysteine peptidase [ PDB-ID 1R75 ]
	Lmaj004144AAA Leishmania major Probable kinase [ PDB-ID 1Y63 ]
	Ldon001686AAA Leishmania donovani Putative mitochondrial associated ribonuclease (90% sequence identity to L. tarentolae MAR1) High structural homology to N-carbamoylsarcosine amidohydrolase (15% sequence identity) including active site Cys [ PDB-ID 1X9G ]
	Tcru003547AAA Trypanosoma cruzi Ribonuclease MAR1 Ribbon diagram showing bound sulfate and large unknown ligand courtesy of E coli (mFo-Fc density at 4 and 7 sigma) [ PDB-ID 1YZV ]
	DBsf000001AYE Engineered protein designed to mimic fold of 1AYE [ PDB-ID 1VJQ ]

Additional Pathogenic Protozoan Structures Solved by MSGPP Members

Plasmodium falciparum
	Myosin A tail domain interacting protein (MTIP) Plasmodium falciparum. [ PDB-ID 2AUC ] Yellow: the closed P. falciparum structure, Blue: the "open" P. knowlesi structure in complex with the MyoA tail helix in red. Bosch J, Turley S, Daly TM, Bogh SM, Villasmil ML, Roach C, Zhou N, Morrisey J.M., Vaidya A.B., Bergman L.W., Hol W. G. Proc Natl Acad Sci U S A (2006) 103:4852-4857 [ Medline ]
	Peptide Deformylase Plasmodium falciparum [ PDB-ID 1JYM ] Abhinav Kumar, Kiet T. Nguyen, Sumant Srivathsan, Brad Ornstein, Stewart Turley, Irwin Hirsh, Dehua Pei and Wim G. J. Hol Structure (2002) 357-367 [ Article ]
	Fructose-1,6-bisphosphate Aldolase Plasmodium falciparum. [ PDB-ID 1A5C ] Kim, H., Certa, U., Dobeli, H., Jakob, P., Hol, W. G. Biochemistry (1998) 37:4388 [ Medline ]
Plasmodium knowlesi
	Myosin A tail domain interacting protein (MTIP) Plasmodium knowlesi. [ PDB-ID 2QAC ] Bosch J, Turley S, Roach CM, Daly TM, Bergman LW, Hol WG. J Mol Biol. (2007) Jun 9; [Epub ahead of print] Yellow: the closed P. falciparum structure, Blue: the "open" P. knowlesi structure in complex with the MyoA tail helix in red. [ Medline ]
Leishmania mexicana
	Glyceraldehyde 3-Phosphate Dehydrogenase Leishmania mexicana [ PDB-ID 1GYP ] Kim, H., Feil, I. K., Verlinde, C. L., Petra, P. H., Hol, W. G. Biochemistry (1995) 34:14975 [ Medline ]
	Glycerol-3-phosphate Dehydrogenase Leishmania mexicana [ PDB-ID 1EVY ] Suresh, S., Turley, S., Opperdoes, F. R., Michels, P. A. M., Hol, W. G. J. Structure (2000) 8:541 [ Medline ]
	Fructose-1,6-bisphosphate Aldolase Leishmania mexicana Glycosomal Fructose-1,6-bisphosphate Aldolase [ PDB-ID 1EPX ] Chudzik, D. M., Michels, P. A., De Walque, S., Hol, W. G. J. J.Mol.Biol. (2000) 300:697 [ Medline ]
Leishmania donovani
	Heterodimeric Topoisomerase 1 Leishmania donovani [ PDB-ID 2B9S ] Davies, D.R., Mushtaq, A., Interhtal, H., Champoux, J.J. & Hol, W. G. J. (2006) J Mol Biol 357, 1202-1210
Trypanosoma brucei
	RNA editing ligase I Trypanosoma brucei [ PDB-ID 1XDN ] Deng, J., Schnaufer, A., Salavati, R., Stuart, K. & Hol, W. G. J. (2004) J Mol Biol 343, 601-613
	RNA editing TUTase 2 Trypanosoma brucei [ PDB-ID 2B4V ] [ PDB-ID 2B51 ] [ PDB-ID 2B56 ] Deng, J., Lewis Ernst, N., Turley, S., Stuart, K. & Hol, W. G. J. (2005) EMBO J. 24, 4007-4017
	Peroxin 5 Fragment Trypanosoma brucei Third Tpr Motif of the Peroxin Pex5 [ PDB-ID 1HXI ] Kumar, A., Roach, C., Hirsh, I. S., Turley, S., Dewalque, S., Michels, P. A. M., Hol, W. G. J. J.Mol.Biol. (2001) 307:271 [ Medline ]
	Phosphoglycerate Kinase Trypanosoma brucei Phosphoglycerate kinase [ PDB-ID 16PK ] Bernstein, B. E., Michels, P. A. M. & Hol, W. G. J. (1997) Nature 385, 275-278 [ Medline ]
	Fructose-1,6-bisphosphate Aldolase Trypanosoma brucei [ PDB-ID 1F2J ] Chudzik, D. M., Michels, P. A., De Walque, S., Hol, W. G. J. J.Mol.Biol. (2000) 300:697 [ Medline ]
	Glyceraldehyde-3-phosphate Dehydrogenase Trypanosoma brucei [ PDB-ID 1GGA ] Vellieux, F. M., Hajdu, J., Verlinde, C. L., Groendijk, H., Read, R. J., Greenhough, T. J., Campbell, J. W., Kalk, K. H., Littlechild, J. A., Watson, H. C., et al. Proc Natl Acad Sci USA (1993) 90:2355 [ Medline ]
	Triosephosphate Isomerase Trypanosoma brucei [ PDB-ID 5TIM ] Rik K. Wierenga, Kor H. Kalk and Wim G. J. Hol Journal of Molecular Biology (1987) 198:109-121